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. 2021 Apr 7;16(1):558–569. doi: 10.1515/med-2021-0209

Figure 4.

Figure 4

Effects of miR-330-5p inhibitor on ox-LDL-induced HUVECs injury. HUVECs were transfected with si-NC, si-circ_0003204, si-circ_0003204 + in-miR-NC, or si-circ_0003204 + in-miR-330-5p, followed by treatment with ox-LDL. (a) The expression of miR-330-5p was measured using qPCR. (b) CCK8 assay was performed to test the viability of HUVECs. (c) The apoptosis of HUVECs was determined via flow cytometry. (d) Caspase-3 Activity Assay Kit was used to assess the activity of caspase-3. (e–g) The contents of IL-6, IL-1β, and TNF-α were detected using the IL-6, IL-1β, and TNF-α ELISA Assay Kits, respectively. (h–j) The ROS, MDA, and LDH Assay Kits were used to measure the expression of ROS and MDA and the activity of LDH. *P < 0.05.