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. 2021 Apr 7;16(1):558–569. doi: 10.1515/med-2021-0209

Figure 5.

Figure 5

TLR4 was a target of miR-330-5p. (a) The sequences of TLR4 3′UTR contained the binding sites or mutant binding sites of miR-330-5p were presented. (b and c) The luciferase activity of TLR4 3′UTR WT or MUT vector in ox-LDL-induced HUVECs was measured using dual-luciferase reporter assay. (d) The interaction between TLR4 and miR-330-5p was confirmed via RNA pull-down assay, and the relative expression of TLR4 in Bio-miR-330-5p probe or Bio-miR-330-5p MUT probe was determined using qPCR. (e) The protein level of TLR4 in ox-LDL-induced HUVECs or normal HUVECs was detected by WB analysis. (f) The protein level of TLR4 was tested using WB analysis to evaluate the effect of miR-330-5p mimic on TLR4 expression. (g) HUVECs were transfected with si-NC, si-circ_0003204, si-circ_0003204 + in-miR-NC, or si-circ_0003204 + in-miR-330-5p, followed by treatment with ox-LDL. WB analysis was performed to assess the expression of TLR4. *P < 0.05.