Skip to main content
. 2021 Apr 7;16(1):558–569. doi: 10.1515/med-2021-0209

Figure 6.

Figure 6

Effects of miR-330-5p mimic and TLR4 overexpression on ox-LDL-induced HUVECs injury. HUVECs were transfected with miR-NC, miR-330-5p, miR-330-5p + pcDNA, or miR-330-5p + TLR4, followed by treatment with ox-LDL. (a) The protein level of TLR4 was determined using WB analysis. (b) The viability of HUVECs was detected via CCK8 assay. (c) The apoptosis of HUVECs was measured using flow cytometry. (d) Caspase-3 Activity Assay Kit was used to determine the activity of caspase-3. (e–g) The IL-6, IL-1β, and TNF-α ELISA Assay Kits were used to detect the contents of IL-6, IL-1β, and TNF-α, respectively. (h–j) The expression of ROS and MDA and the activity of LDH were assessed using the ROS, MDA, and LDH Assay Kits, respectively. *P < 0.05.