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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2279:59–73. doi: 10.1007/978-1-0716-1278-1_6

Fig. 2.

Fig. 2.

Final embedding set-up of the cell spheroid to obtain the paraffin block that will be subsequently cut. The block is put again in molten paraffin (left), and before the paraffin solidifies the O-ring or an embedding cassette without the lid is placed (center), and the whole set-up is allowed to solidify to create the final block that will be cut in the microtome (right).