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Steps for the dehydration and clearing of cell 3D spheroids formed from cell pellets. RT, room temperature.
| Procedure | Steps | Solution | Time/Temperature |
|---|---|---|---|
| Dehydration | 1 | 70% alcohol | 15 min/RT |
| 2 | 80% alcohol | 15 min/RT | |
| 3 | 95% alcohol | 15 min/RT | |
| 4 | 100% alcohol | 15 min/RT | |
| Clearing | 1 | Xylene | 10min/RT |
| 2 | Xylene | 10min/RT | |
| 3 | Xylene | 10min/RT | |
| 4 | Xylene | 10min/RT |
