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. 2021 Mar 26;11:608201. doi: 10.3389/fonc.2021.608201

Figure 3.

Figure 3

Verification of the expression pattern of the selected protein targets identified by GeLC-MS/MS with Western blot and Immunofluorescence (IF) analysis. (A, B) Consistent with the proteomic results, Western blot detection of HER2 and STEAP4 in BC cell lines was only found in HCC-1954 (HER2+) cells. IF qualitative and relative quantitative results of HER2 and STEAP4 confirmed their overexpression in HCC-1954 cells as compared to the control MCF-10A and the MDA-MB-231 (TNBC) cells (***p < 0.001). (C) EPHA2 was highly expressed in MDA-MB-231 and HCC-1954 but faintly detectable in the control MCF-10A cells by Western Blot and IF qualitative and quantitative analysis, as we expected from the proteomic results (***p < 0.001). (D) CD97 expression was only detected in MDA-MB-231 (TNBC) through Western blot and IF results (**p < 0.01, ***p < 0.001). Scale bars in the IF images represent 40 µm.