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. 2021 Mar 26;11:608201. doi: 10.3389/fonc.2021.608201

Figure 6.

Figure 6

Effect of STEAP4 silencing on cell proliferation and Lapatinib inhibition in HCC-1954 (HER2+) cells. (A) Knockdown efficiency of the three siRNA duplexes on STEAP4 protein expression in HCC-1954 cells, as assessed by Western blot analysis 48 h after transfection with 10nM siRNAs. (B) HCC-1954 cell growth curves at 24, 48, and 72 h after transfection with 10nM siRNAs. Cell proliferation OD values were detected by MTT assay. STEAP4 A-siRNA and B-siRNA significantly reduced cell proliferation at 72 h of transfection as compared to the NC-siRNA (non-silencing of STEAP4) or Control group (**p < 0.01). (C) Dose–response curves and the IC50 values after siRNA and Lapatinib treatment. HCC-1954 cells were treated with a fixed concentration of siRNAs (10 nM) and different concentrations of Lapatinib for 72 h to assess the effect of A-siRNA and Lapatinib on cell viability using the MTT assay. NC-siRNA (non-silencing of STEAP4) was used as the negative control for comparison (p < 0.01). The results represent the mean values ± SD of three independent experiments.