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. 2021 Mar 26;12:619392. doi: 10.3389/fimmu.2021.619392

Figure 2.

Figure 2

MRNA deep sequencing results and validation after gain of miR-424 and miR-497 function for 48 h in RASF. (A) The expression of miR-424 and miR-497 in RASF transfected with 10 nM mimic 48h detected by using RT-qPCR. (B) Heat map of all dysregulated genes from mRNA deep sequencing from 10nM miR-424 or miR-497 mimic-treated RASF cells. (C) Venn diagram of dysregulated genes in miR-424 and miR-497 mimic-treated cell from miRNA deep sequencing results. (D, E) RT-qPCR results of dysregulated genes in miR-424 mimic-treated cells (D) and miR-497 mimic-treated cells (E). (F) KEGG signal pathway analysis diagram. RASF cells isolated from synovial tissue of 4 RA patients were transfected with miR-424 or miR-497 mimics and inhibitors, respectively. Triplicates were used for each transfection experiment. Bar: mean ± SEM of samples from 4 patients. P<0.05, *: vs. NC mimic, using Mann-Whitney U test.