Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
MCF10A (Homo sapiens)	Brugge laboratory
Recombinant DNA
pMB‐PB‐FoxO3A‐mNeonGreen	Pertz laboratory
pHygro‐PB‐ERK‐KTR‐mTurquoise2	Pertz laboratory
pPBbSr2‐miRFP703	Pertz laboratory
Chemicals, enzymes, and other reagents
Recombinant Human IGF‐I	PeproTech	100‐11
Recombinant Human Betacellulin	PeproTech	100‐50
Recombinant Human Epiregulin	PeproTech	100‐04
Animal‐Free Recombinant Human EGF	PeproTech	AF‐100‐15
Human HGF	PeproTech	100‐39‐10UG
Animal‐Free Recombinant Human Heregulinβ‐1	PeproTech	AF‐100‐03
Insulin solution human	Sigma‐Aldrich	I9278‐5ML
Hydrocortisone	Sigma‐Aldrich	H0888‐1G
Horse serum Donor Herd	Sigma‐Aldrich	H1270‐500ML
DMEM/F12 Ham	Sigma‐Aldrich	D6434‐500ML
Penicillin/Streptomycin	Sigma‐Aldrich	P4333
BSA	Sigma‐Aldrich	A2153
FuGENE® HD Transfection Reagent	Promega	E2311
Hygromycin B solution	Santa Cruz biotechnology	sc‐29067
Blasticidin S HCl	Tocris Bioscience	5502
Puromycin dihydrochloride	Sigma‐Aldrich	P7255
Software
Ilastik	v1.3.2
Cell profiler	v3.1.8
MATLAB	R2016b
Other
Eclipse Ti inverted fluorescence microscope	Nikon
Zyla 4.2 plus camera	Andor