Table 1. Primer sequences of qPCR analysis in the research.
| Gene name | Gene ID | Protein name | Primer pairs |
|---|---|---|---|
| Csa_6G499090 | 101204542 | Mitochondrial dicarboxylate carrier protein | F: 5′- GGGCCAATGGCACTTTACAA-3′ R: 5′- TGTTCCAGCGTCACAAACAG-3′ |
| nad5 | 11123919 | NADH-ubiquinone oxidoreductase chain 5 | F: 5′- TTGCTTGCGGCATCTCTAAC-3′ R: 5′- CCGCATATCTTGCTCATCCG-3′ |
| Csa_7G419610 | 101214617 | S-adenosylmethionine synthase | F: 5′- CCTTGTACCGTTGAGCTTCG-3′ R: 5′- ATCGGCAGCGTAGATCTGAA-3′ |
| Csa_3G099680 | 101207278 | Chlorophyll a-b binding protein | F: 5′- CAAGCCATTGTGACCGGAAA-3′ R: 5′- TTGGTGGCATAAACCCAAGC-3′ |
| atpI | 3429379 | ATP synthase subunit a | F: 5′- TTGCTCACGTCTCGAATGAA-3 R: 5′- GGAAGTCGGCCAACATTTGTA-3′ |
| Csa_5G457770 | 101222001 | Aspartokinase | F: 5′- TCAGGTCCTGCATCCACAAT-3′ R: 5′- CTCTGTTCCGGGTGATGAGA-3′ |
| Csa_6G517340 | 105434554 | DNA-directed RNA polymerase subunit beta | F: 5′- CACACTTCCTCCGTTACCCT3′ R: 5′- CCCAAGCATCTTCCTGTGTG-3′ |
| Csa_3G873780 | 101213650 | Zinc finger protein | F: 5′- GATGACGGTTATGGCGATGG-3′ R: 5′- CAGCAGACCATGTCGAAGAC-3′ |
| Csa_2G248700 | 101208904 | Glucose-1-phosphate adenylyltransferase | F: 5′- AGCAACTGCATCAACAGTGG-3′ R: 5′- GCTGCAAGAACCTCCACAAA-3′ |
| Csa_2G258680 | 101211191 | 40S ribosomal protein S12 | F: 5′- CGGTTCGATCGGTTTCTACG-3′ R: 5′- TGTCCATTGGCTCACCAAGA-3′ |
| Csa_7G343850 | 101221055 | Pectinesterase | F: 5′- TTGCCTTCTTCTTCCCTGGT-3′ R: 5′- AGCTGAGCATTCTTCTCCGA-3′ |
| Csa_7G253750 | 101214097 | Glycosyltransferase | F: 5′- TCCAAGATTGGGACTGCCAT -3′ R: 5′- TCACGGAACGAGAATCACGA -3′ |
| Csa_5G630860 | 101209617 | Folylpolyglutamate synthase | F: 5′- TGTCTCCGTTGAAGCCAAAC-3′ R: 5′- GTAGCTGTTGGACGCTACAC-3′ |
| Csa_6G510320 | 101214692 | Hexosyltransferase | F: 5′-GAGCCCGTTGCGATTGTTTA-3′ R: 5′-ACGCTCTATGACACCTTGGA-3′ |
| Csa_1G542510 | 101213646 | Phloem protein | F: 5′- GGGAATTCAAGGTCGACAAACA-3′ R: 5′-TAGTGGGAGTGGGAGTGAG-3′ |
| Lec26 | 101213646 | 26 kDa phloem lectin | F: 5′-GGGAATTCAAGGTCGACAAACA-3′ R: 5′-TAGTGGGAGTGGGAGTGAGA-3′ |
| Csa_1G710160 | 105436367 | Phloem filament protein | F: 5′-AGCAGCAAACGACAAAGGAG-3′ R: 5′-CAAACTTTGCCACGTCTTGC-3′ |
| Csa_2G172500 | 101207168 | Methionine aminopeptidase | F: 5′-CAAATGAGGGCTGCTTGTCA-3′ R: 5′-CGTAGCCAAGAGGTGAAGGA-3′ |
| Csa_5G633260 | 101223124 | Glycosyltransferase | F: 5′-GACCAACGAATCCGCTTCAA-3′ R: 5′- CTCATCCTCCGACTGACCTC -3′ |
| Csa_6G426880 | 101220980 | Serine/threonine-protein phosphatas | F: 5′- ATTGAGCGGATGGGAGAGAG-3′ R: 5′- AAAGCTGCTAGAGGAAGCCA-3′ |
| Csa_2G421020 | 101213629 | Peroxidase | F: 5′- CTGAGAGGGATTCTGCACCA-3′ R: 5′-ATCTCTTCGGCCAGTTGGAA -3′ |
| ClCa | 101210008 | Chloride channel protein | F: 5′-GATTCCCTGTTGTGGATGCC-3′ R: 5′- AGTACTAGTCCGTGCAGCTC-3′ |
| Csa_3G166240 | 101211228 | DNA-directed RNA polymerase subunit | F: AAGCTCTTGTGTTCGGCTTG-3′ R: GCTAGGGCTTTAGGAGCTGA-3′ |
| ACO1 | 101221653 | 1-Aminocyclopropane-1-carboxylate oxidase | F: 5′- AGCCAGCAAAGGATTGAACG-3′ R: 5′- CGATGTTGGAGACTGGGAGA-3′ |
| ACS2 | 101217331 | ACC synthase | F: 5′- TTCTCCTCCGACGAGTTCAC-3′ R: 5′- CGGTGGTGACGACTTTATCG-3′ |
| ETR1 | 101213479 | Ethylene receptor | F: 5′- GTGCTAGACAATGGCGTGTT-3′ R: 5′- CTCTGCTTCTCTTCGGGCTA-3′ |
| ERS | 101205786 | Ethylene response sensor | F: 5′- GCTGTTGCACTTTCACATGC-3′ R: 5′- CTCTGCTTCTCTTCGGGCTA-3′ |
| rbcS | 101219300 | Ribulose-1, 5-bisphosphate carboxylase/oxygenase small subunit | F: 5′- GCCTCTCAGACTCAACACCA-3′ R: 5′- CGGAAGATTTGAGGCCAGTG-3′ |
| GAPDH | 101202856 | Glyceraldehyde-3-phosphate dehydrogenase | F: 5′- GAAGCACATTGAGGCTGGAG-3′ R: 5′- GACTCGTCATGGCTGTATGC-3′ |
| FBA | 101219476 | Fructose-1,6-bisphosphate aldolase | F: 5′- AAGCGACTGGCAAGCATAAG-3′ R: 5′- TTGAAGCACTTCCACGAACG-3′ |
| TK | 101215805 | Transketolase | F: 5′- ATGCAATGGGATTGCCCTTC-3′ R: 5′- TGGGTTGGACCATCTTCTCC-3′ |
| actin | DQ641117 | Actin | F: 5′-CCCATCTATGAGGGTTACGCC-3′ R: 5′-TGAGAGCATCAGTAAGGTCACGA-3′ |