Figure 4.

The ability of PCBP1 to regulate circadian rhythm is conserved in evolution. (A) The expression of the PCBP1 gene is rhythmic in the mouse heart. The phase of PCBP1 is ZT16, which is similar with PER2 (ZT14) and CRY1 (ZT20), but contrary to BMAL1 (ZT24). All data are presented as the mean ± SEM from at least four independent experiments. The qPCR data were analyzed by JTK_Cycle analysis to determine the rhythmicity and the phases of the peaks (time course threads with both p and q values less than 0.05, were considered as rhythmic). (B) Drosophila with conditional knockdown of mushroom-body expressed (mub), the homolog gene of PCBPs in Drosophila, in circadian neurons show a period-lengthening circadian rhythm (middle) compared to wild-type (left). Right: Bars show quantitative period change values Statistical significance was determined using Student’s t-tests (n ≥ 14, ^**p < 0.01).