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. Author manuscript; available in PMC: 2021 Apr 9.
Published in final edited form as: J Vis Exp. 2020 Dec 4;(166):10.3791/61967. doi: 10.3791/61967

Figure 4. SEM analysis of bladders fixed in situ.

Figure 4.

Bladders were collected from mice 3 h after two exposures (12 h apart) to PBS (A) or G. vaginalis (C). Dotted lines illustrate a single urinary epithelial cell, which is smaller in G. vaginalis-exposed bladders because the large superficial cells have exfoliated away revealing the underlying transitional epithelium. (B) Bladder collected 6 h after initial inoculation with UPEC, during Phase 1 of the model, showing urothelial exfoliation and extracellular UPEC. (D) Example of insoluble fat droplets present on the bladder surface. Scale bars are 20 μm in the main images and 2 μm in the inset.