FIGURE 1.

H₂S pretreatment reduced LPS + H₂O₂ induced injury in testosterone synthesis of MLTC‐1 cells. The model + H₂S group was pretreated with H₂S donor GYY4137 (50 μmol/L) for 4 h, and then added with LPS (10 μg/mL) + H₂O₂ (250 μmol/L) for 12 h. The model group was pretreated with PBS and treated with LPS (10 μg/mL) + H₂O₂ (250 μmol/L) for 12 h, and the control group was incubated with PBS only. (A) The cell viability of each group was detected using CCK‐8 method at 0 h and 24 h. (B) The concentration of H₂S and testosterone were examined using ELISA kits. (C) The levels of proteins related to testosterone synthesis were detected by western blotting. (D) The mRNA levels of genes related to testosterone synthesis were detected by RT‐qPCR. *P < .05, *P < .01 compared with control group, NS represented no significance