FIGURE 2.

CBS overexpression inhibited while knockdown promoted LPS + H₂O₂ induced injury in testosterone synthesis of MLTC‐1 cells. The model group was added with LPS (10 μg/mL) + H₂O₂ (250 μmol/L) for 12 h, and the control group was incubated with PBS only. (A) The expression of CBS mRNA in each group treated with indicated vector transfection was detected by RT‐qPCR. (B) The cell viability of each group was detected using CCK‐8 method at 0 h and 24 h. (C) The concentration of H₂S and testosterone were examined using ELISA kits. (D) The levels of proteins related to testosterone synthesis were detected by western blotting. (E) The mRNA levels of genes related to testosterone synthesis were detected by RT‐qPCR. *P < .05, *P < .01 compared with control group, #P < .05, #P < .01 compared with model group, NS represented no significance