FIGURE 4.

CBS overexpression in vivo inhibited LPS + H₂O₂ induced injury in testosterone synthesis through activating cAMP/PKA pathway. (A) The concentration of H₂S and testosterone were examined using ELISA kits. (B) PAS staining was used to detect the inflammatory status of testis in each group. (C) The levels of CBS, p‐PKA and PKA proteins expression were detected by western blotting. (D) The activity of cAMP was examined using ELISA kits. (E) The levels of relative mRNAs related to testosterone synthesis were detected by RT‐qPCR. *P < .05, *P < .01 compared with control group, #P < .05, #P < .01 compared with model group, NS represented no significance