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. 2021 Mar 6;25(7):3226–3238. doi: 10.1111/jcmm.16392

FIGURE 2.

FIGURE 2

Circ‐LAMP1 promotes CCA cell progression in vitro. A, qRT‐PCR for circ‐LAMP1 expression after silencing of circ‐LAMP1 in KMBC cells. B, qRT‐PCR for circ‐LAMP1 expression after up‐regulation of circ‐LAMP1 in RBE cells. C, Cell viability was detected by CCK‐8 after silencing of circ‐LAMP1 in KMBC cells. D, Cell viability was detected by CCK‐8 after up‐regulation of circ‐LAMP1 in RBE cells. E, Clone forming capacity was detected by colony formation assay after silencing of circ‐LAMP1 in KMBC cells. F, Clone forming capacity was detected by colony formation assay after up‐regulation of circ‐LAMP1 in RBE cells. G, Cell apoptosis was detected by flow cytometric assay after silencing of circ‐LAMP1 in KMBC cells. H, Cell apoptosis was detected by flow cytometric assay after up‐regulation of circ‐LAMP1 in RBE cells. I, Cell migration was detected by wound healing assay after silencing of circ‐LAMP1 in KMBC cells; Scale bars = 200 μm. J, Cell migration was detected by wound healing assay after up‐regulation of circ‐LAMP1 in RBE cells; Scale bars = 200 μm. K, Cell migration and invasion was detected by transwell assay after silencing of circ‐LAMP1 in KMBC cells; Scale bars = 100 μm. L, Cell migration and invasion was detected by transwell assay after up‐regulation of circ‐LAMP1 in RBE cells; Scale bars = 100 μm. *P < .05, **P < .01