FIGURE 4.

Anti‐HCV effects are eliminated by residue mutations in TGF‐β isoforms, but not influenced by deletion of α‐helix 3. Huh7.5 cells transfected with plasmids with His tag at C‐terminal (A), C77S mutant (B), C109Y mutant (C), TGF‐β α‐helix 3 deletion, or R25K, V92I and R94K mutants (D) for 48 h were sub‐cultured and then infected with HCV after 24 h. Intracellular HCV core was detected by WB after 48 h. Data represent the mean ± SD of three independent experiments. ANOVA analysis followed by the Student's t test method was used. *P < 0.05, **P < 0.01 and ***P < 0.001 vs plasmid control