TABLE 1.
The oligonucleotide sequences used in PCR or qRT‐PCR
| Gene | Sequence (5'→3') | |
|---|---|---|
| For plasmid | ||
| TGF‐β1 | S | CCGGAATTCCGGATGCCGCCCTCCGGGCTGCG |
| R | GCTCTAGAGCTCAGCTGCACTTGCAGGAGC | |
| TGF‐β3 | S | CCCAAGCTTATGAAGATGCACTTGCAAAGGGCTC |
| R | CCGCTCGAGTCAGCTACATTTACAAGACTTCACCACCA | |
| TGF‐β1‐His | S | GGAATTCCATGCCGCCCTCCGGGCTGCG |
| R | CTCTAGATCAATGGTGATGGTGATGATGGCTGCACTTGCAGGAGCGCA | |
| TGF‐β3‐His | S | CCCAAGCTTATGAAGATGCACTTGCAAAGGGCTC |
| R | CCGCTCGAGTCAATGGTGATGGTGATGATGGCTACATTTACAAGACTTCACCACCA | |
| TGF‐β1△α3‐His | S1 | GCACAGTGGCGGCCGCATGCCGCCCTCCGGGCTGCG |
| R1 | GCCGAGGCAATGTAGGGGCAGGGCCCGAGGCAGAAG | |
| S2 | CCTACATTGCCTCGGCGGCGCCGTGCTGCGTGCCGC | |
| R2 | GGCCCTCTAGACTCGATCAATGGTGATGGTGATGATGGCTGCACTTGCAGGAGCG | |
| TGF‐β3△α3‐His | S1 | GCACAGTGGCGGCCGCATGAAGATGCACTTGCAAAG |
| R1 | GCAGATGCGAGGTATGGGCAAGGGCCTGAGCAGAAG | |
| S2 | CATACCTCGCATCTGCCTCGCCTTGCTGCGTGCCCC | |
| R2 | GGCCCTCTAGACTCGATCAATGGTGATGGTGATGATGGCTACATTTACAAGACTTC | |
| For qRT‐PCR | ||
| HCV | S | CGGGAGAGCCATAGTGGTCTGCG |
| R | CTCGCAAGCACCCTATCAGGCAGTA | |
| P | FAM‐5′‐AGGCCTTGTGGTACTGCCT‐3′‐TAMRA | |
| GAPDH | S | CGGAGTCAACGGATTTGGTCGTAT |
| R | AGCCTTCTCCATGGTGGTGAAGAC | |
| P | FAM‐5′‐CCGTCAAGGCTGAGAACGG‐3′‐TAMRA | |
| TGF‐β1 | S | GCAGCACGTGGAGCTGTA |
| R | CAGCCGGTTGCTGAGGTA | |
| TGF‐β2 | S | CCTTCTTCCCCTCCGAAAC |
| R | AGAGCACCTGGGACTGTCTG | |
| TGF‐β3 | S | CGCAGTGCAGACACAA |
| R | TGCTTCAGGGTTCAGA | |
| COL1A1 | S | TCTGGCGCTCCCATGGCTCT |
| R | GCCCTGCGGCACAAGGGATT | |
| α‐SMA | S | GAGTTACGAGTTGCCTGATGG |
| R | GATGCTGTTGTAGGTGGTTTCA | |
Abbreviations: P, probe; R, reverse complementary sequence; S, sense sequence.