FIGURE 6.

PDI sulfhydration sites and their activity. A, Two conserved cysteine‐terminal CXXC in humans, rat and mouse. B, H₂S induced PDI sulfhydration changes by transfection wild‐type human PDI (pPDI) plasmid, mutation C53/57 (M1) plasmid, mutation 397/400 plasmid (M2) and mutation four cysteine site (M3) plasmid into HEK‐293 cells. C, H₂S‐promoted PDI activity changes while mutation sulfhydration sites of PDI. D, H₂S reduced HHcy‐induced ER stress while mutation sulfhydration sites of PDI. E, The relative protein expression of GRP78, XBP1s and cleaved‐caspase‐12. **P < 0.01, *P < 0.05. F, Schematic diagram of the present findings