Figure 1. Tae2 and Dae2 Enzymes Have Divergent Structural Features.

(A) Ribbon diagram Tae2 from Salmonella enterica Typhi (Tae2^St). Canonical papain-like secondary structures are labeled (α1–4, β1–6, Loop1–3 [L1–3]), and subdomains are colored (gray, orange). Catalytic residues are denoted (labeled His 73 and Cy-s23Ala, red).

(B) Structural alignment of Tae2^St (gray) and a Tae2-based homology model of Dae2^Is (golden). Loops 1, 2, and 3 are colored red in Dae2^Is.

(C and D) Electrostatic charges in surfaces proximal to the catalytic residues for Tae2^St and Dae2^Is (scale is in kcal mol⁻¹e⁻¹). The loops are boxed in sequence alignment in Figure S1B.

See also Figure S2 and Table S1.