Ticks Resist Skin Commensals with Immune Factor of Bacterial Origin

. Author manuscript; available in PMC: 2021 Dec 10.

Published in final edited form as: Cell. 2020 Dec 10;183(6):1562–1571.e12. doi: 10.1016/j.cell.2020.10.042

KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Rabbit αDae2^Is antibody	Chou et al., 2015	N/A
Rabbit αTae1^Pa antibody	Chou et al., 2012	N/A
Donkey αRabbit Alexa Fluor 647	Invitrogen	Cat.# A31573
Goat αRabbit-HRP	Advansta	Cat.# R-05072-500

Bacterial and Virus Strains

Escherichia coli (XL1Blue, BL21 (DE3) and Rosetta2 (DE3))	MacroLab	N/A
Bacillus subtilis 168	Carol Gross lab (UCSF)	N/A
Staphylococcus epidermidis (BCM060 and SK135)	Tiffany Scharschmidt lab (UCSF)	N/A
Staphylococcus hominis (SK119)	Tiffany Scharschmidt lab (UCSF)	N/A
Borrelia burgdorferi S9	Patricia Rosa lab (NIH)	N/A

Chemicals, Peptides, and Recombinant Proteins

Recombinant protein Tae2^St	This paper	UniProt: Q8Z969
Recombinant protein Dae2^Is	This paper	Locus: XP_002434728

Critical Commercial Assays

Ambion Silencer siRNA Construction Kit	Ambion	Cat.# AM1620

Deposited Data

Tae2^St protein crystal structure	This paper	PDB: 6WIN (https://www.rcsb.org/structure/6win)

Experimental Models: Organisms/Strains

Mouse: C3H/HeJ	Jackson laboratories	Cat.# 000659

Oligonucleotides

Table S2	This paper	N/A

Recombinant DNA

Overexpression construct pET29b::Tae2^St	Russell et al., 2012	N/A
Overexpression construct pET29b::Dae2^Is	Chou et al., 2015	N/A

Software and Algorithms

Phenix	NIH General Medical Sciences	https://www.phenix-online.org
Molecular Operating Environment	Chemical Computing Group	https://www.chemcomp.com
Maestro	Schrodinger	https://www.schrodinger.com