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. 2021 Jan 20;5(4):661–674. doi: 10.1002/hep4.1656

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© 2021 The Authors. Hepatology Communications published by Wiley Periodicals LLC on behalf of the American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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FIG. 2 — TEAD4 overexpression increases tumor growth in vivo. (A) Schematic representation of the CAM assay. (B) Photographs of TEAD4‐overexpressing or control Huh7 cells implanted in CAMs and grown for 4 days postimplantation. (C) Volume of tumors derived from the CAM experiment (n ≥ 7 tumors over two independent experiments). Values are normalized to the mean volume of control. (D) TEAD4 expression in Huh7 tumors extracted 4 days postimplantation analyzed by western blot (left) and IHC (right). Tumoral cells were immunostained with the TEAD4 antibody. Scale bars, (B) 1 cm and (D) 50 μm and 20 μm. Statistical significance was determined by the Mann‐Whitney U test; *P < 0.05. Data are mean ± SD. Abbreviations: H&E, hematoxylin and eosin; ox, overexpression.