FIG. 3.

TEAD4 regulates cell differentiation and proliferation and the expression of HSP70 family genes. (A) Heatmap showing the top 50 differentially expressed genes in TEAD4‐overexpressing Huh7 cells. (B) Volcano plot of –log10 adjusted P value against log2 fold change to illustrate the differentially expressed genes between TEAD4‐overexpressing Huh7 cells and control cells. Red dots indicate differentially expressed genes (adjusted P < 0.05). (C‐E) GSEA plots show selected significantly enriched gene sets in TEAD4‐overexpressing cells: Hallmark gene sets from (C) MSigDB database, (D) HSP family gene set, and (E) GO gene sets from the MSigDB database. (F) The Huh7 cell line was transiently transfected with a vector overexpressing TEAD4 and the corresponding control. RNA was extracted at different time points. mRNA expression levels of HSPA1A (left) and HSPA6 (right) were measured by qRT‐PCR using GAPDH as the internal control. Data are shown as fold change relative to control. Results represent three independent experiments. Statistical significance was determined by the Mann‐Whitney U test; *P < 0.05, **P < 0.01, ***P < 0.001. Data are mean ± SD. Abbreviations: E2F, E2 factor; FDR, false discovery rate; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; LATS1, large tumor suppressor kinase 1; mRNA, messenger RNA; mTORC1, mammalian target of rapamycin complex 1; NFKB, nuclear factor kappa B; ox, overexpression; TJP2, tight junction protein 2; TNFa, tumor necrosis factor alpha; UV, ultraviolet.