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. 2021 Jan 20;5(4):661–674. doi: 10.1002/hep4.1656

FIG. 5.

FIG. 5

The effect of TEAD4 on cell proliferation is independent of expression of cofactors YAP and TAZ. (A) Schematic representation of TEAD4 transcriptional activity associated with YAP/TAZ cofactor expression. (B) Expression levels of YAP and TAZ were measured in stable YAP/TAZ‐knockdown (shY/T) and control (shCTR) Huh7 and SNU449 cell lines by qRT‐PCR normalized to GAPDH as internal control. (C) TEAD4 expression in stably TEAD4‐overexpressing and control Huh7 and SNU449 cell lines with knockdown of YAP/TAZ or control (shCTR). (D) Proliferation kinetics of stable TEAD4‐overexpressing and control Huh7 cell lines with YAP/TAZ knockdown or control (shCTR) (left) and in TEAD4‐overexpressing and control SNU449 stable cell lines with YAP/TAZ knockdown and control (shCTR) (right). (E) HSPA6 and HSPA1A expression levels in TEAD4‐overexpressing and control Huh7 cell lines with knockdown of YAP/TAZ or control (shCTR) were measured by qRT‐PCR using GAPDH as reference. (F) Expression levels of HSPA6 in TEAD4‐overexpressing and control Huh7 cell lines with knockdown of YAP/TAZ or control (shCTR) were measured by western blot at 48 and 72 hours posttransfection. Data are shown as fold change to control. Results represent three independent experiments. Statistical significance was determined by t test; *P < 0.05, **P < 0.01, ***P < 0.001. Data are mean ± SD. Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; h, hours; ox, overexpression; sh, short hairpin; VGLL, vestigial‐like protein 1.