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. 2021 Mar 8;49(6):3546–3556. doi: 10.1093/nar/gkab123

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Cas9-integrase supercomplex generates physiologically relevant substrates –CRISPR integration. (A) Schematic of a novel screen developed for the selection of full-site integration products from the type II-A CRISPR-Cas acquisition machinery. R: Repeats; S: Spacers; *S: New spacer (B–G), distribution of spacer lengths for different kinds of prespacers upon full-site integration by Cas1-Cas2 integrase (blue), supercomplex + complementary sgRNA (pink) and supercomplex + non-complementary sgRNA (yellow). Sequence of substrates and population size for each given prespacer and complexes has been listed in Supplementary Tables S2 and S3, respectively.