Skip to main content
. 2021 Mar 3;49(6):3109–3126. doi: 10.1093/nar/gkab130

Figure 8.

Figure 8.

Conservation analysis of the CTD interaction sites on the GTase, comparison to the C. albicans GTase–CTD cocrystal structure, and the palindromic nature of the CTD. (A) CTD interaction sites observed in the C. albicans GTase–CTD cocrystal structure (PDB ID: 1P16) (31). The positions of the N- and C-ter heptads of the CTD are indicated. The residue numbers are from the C. albicans GTase. (B) Multiple sequence alignment of animal and yeast CE GTase sequences in the CDS and allosteric pathway regions. Numbers above indicate the human CE residue numbers and below are the C. albicans residue numbers. The CDS sites are indicated by coloured circles above/below the residue numbers. (C) MMGBSA analysis comparing the CTD binding affinity to the human GTase between the ‘mammalian’ orientation (as in Figure 4; System 6) and the ‘yeast’ orientation (from the C. albicans crystal structure extended to four heptads; System 11; see details in the Materials and Methods). (D) One CTD heptad displayed in the conventional way (above) and then shifted and centred at Tyr1 (below) to illustrate the palindromic nature of the repeating sequence.