Figure 5.

BRCA1-BARD1 regulates DNA-binding of multiple transcription regulators. (A) pActin was incubated in extract that was mock-depleted, BRCA1-depleted, or supplemented with 200 nM BRCA1-BARD1. DNA-bound proteins were isolated by plasmid pull-down and analyzed by mass spectrometry. Results were normalized to ΔMock samples and ranked by enrichment of ΔBRCA1 over +BRCA1-BARD1 samples. Representative complex members are labeled. An extended graph showing distribution of ranked proteins is shown in Supplementary Figure S5. (B) Schematic showing the relationship between BRCA1, BRD4, and complexes identified in (A).