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. 2021 Feb 28;49(6):3263–3273. doi: 10.1093/nar/gkab111

Figure 6.

Figure 6.

BRCA1-BARD1 suppresses H4K8 acetylation and BRD4 binding. (A) pActin was incubated in mock- or BRCA1-depleted extract. At the indicated time points, DNA-bound proteins were isolated by plasmid pull-down and analyzed by western blot. Total recovery of plasmid DNA from mock- and BRCA1-depleted extracts is shown in Supplementary Figure S6. (B) pActin was incubated in extract supplemented with buffer or 200 nM BRCA1-BARD1 and DNA-bound proteins were analyzed as in (A). (C) pActin was incubated in extract supplemented with buffer or JQ1 (BETi). At the indicated time points, DNA-bound proteins were analyzed as in (A). (D) pActin was incubated in extract with increasing amounts of JQ1. After 120 min, RNA was quantified by RT-qPCR (n = 3). (E) pActin was incubated in NPE supplemented with buffer or JQ1. At the indicated time points, RNA was quantified by RT-qPCR. (F) pActin was incubated in mock- or BRCA1-depleted extract. Samples were withdrawn after 20 min and analyzed by ChIP with the indicated antibodies (n = 2). (G) pActin was incubated in extract supplemented with buffer or JQ1. Samples were withdrawn after 20 min and analyzed by ChIP with the indicated antibodies (n = 2).