Figure 4.

The cspB and cspC hairpins from the O conformation could work as thermoresponsive RNA elements. (A) Schematic representation of the MB design that mimics the stem loop of the O conformation from the cspB and cspC 5′UTRs. The 5′ and 3′ ends harbour the FAM and IBkFQ molecules, respectively. Fluorescence emission occurs when the FAM fluorophore is separated from the IBkFQ quencher. (B) Detection of the FAM fluorescence from MB-CspB (red), MB-CspC (green) and MB-CspA (blue) in function of the temperature. The MBs were equilibrated at 13°C for 10 min and then the temperature was increased 3°C every 5 min. After 5 min of incubation at 65°C, the temperature was gradually decreased following the inverse pattern. Fluorescence was registered for the duration of the experiment. Means and standard deviations from three independent replicates are shown.