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. 2021 Feb 22;49(6):e36. doi: 10.1093/nar/gkaa1264

Figure 3.

Figure 3.

Comparing and combining the REDIT method with alternative HDR-enhancing gene-editing approaches. (A) Schematics showing alternative HDR-enhancing approaches via fusing functional domains, CtIP or Geminin (Gem), to Cas9 protein. (Left) Plasmids of individual benchmarks used in panel C; (Right) Plasmids combining the SSAP activity of REDIT with the alternative tools via MS2-aptamer recruitment. (B) Alternative small-molecule HDR-enhancing approach through cell cycle control. Nocodazole was used to synchronize cells at the G2/M boundary (Left), showing experiment procedures and timeline (Right). (C) Comparison of gene-editing efficiencies using REDIT and alternative HDR-enhancing tools, Cas9-HE (CtIP fusion), Cas9-Gem (Geminin fusion), and Nocodazole (noc), along with combination of REDIT with these methods (Cas9-HE/Cas9-Gem/noc+REDIT). Donor DNAs have 200 + 400 bp (DYNLT1) or 200 + 200bp (HSP90AA1) of HAs. All assays performed with no donor, NTC and Cas9 (no enhancement) controls. #P < 0.05, compared to REDIT; ##P < 0.01, compared to REDIT.