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. 2021 Mar 12;49(6):3003–3019. doi: 10.1093/nar/gkab146

Figure 3.

Figure 3.

DUF1127 protein CcaF1 (RSP_6037) affects stress resistance in R. sphaeroides. (A) Growth curves of the wild type or the wild type with the empty vector (EVC) or the plasmids as shown in Figure 2B. All strains were cultivated under microaerobic conditions. The average of three independent measurements and the standard deviation are plotted. The color code for the different strains was also applied in (B) and (C). (B) Zone of inhibition assay of strains overexpressing the plasmids shown in Figure 2B in comparison to the wild type and wild type with empty vector control (pRK4352) under organic peroxide (700 mM tBOOH) and superoxide stress (300 mM paraquat). The plotted values represent the mean of at least three independent experiments and the standard deviation is indicated. (C) Survival assay of strains overexpressing the plasmids shown in Figure 2B in comparison to the wild type and wild type with empty vector control (pRK4352) under organic peroxide stress (300 mM tBOOH). The number of colonies of a control culture grown without the addition of any oxidative stress agents was defined as 100% survival. The bars represent the mean of three independent plating assays and the standard deviation is indicated.