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. 2021 Mar 12;49(6):3003–3019. doi: 10.1093/nar/gkab146

Figure 4.

Figure 4.

RNase E and CcaF1 are involved in CcsR maturation. Analysis of ccaF1-ccsR1–4 and ccsR1–4 precursor and CcsR1 RNA by Northern blot in R. sphaeroides 2.4.1 wild type, rnets mutant and strain 2.4.1 that overexpresses ccaF1 (pRKCcaF1). Cells were harvested at 32°C and at different time points after shift to 42°C. Total RNA was isolated and either run on a 1% formaldehyde agarose gel and, after blotting, hybridized against probes spanning the CcaF1-CcsR1–4 or the CcsR1–4 region, or on a 10% denaturing polyacryamide gel for detection of CcsR1. 5S and 14S rRNA serve as loading controls.