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. 2021 Mar 12;49(6):3003–3019. doi: 10.1093/nar/gkab146

Figure 5.

Figure 5.

CcaF1 binds to CcsR1 and the CcsR1–4 precursor. (A) Gel motility shift RNA-binding experiments with 150 fmol radioactively labelled CcsR1 or CcsR1–4 incubated with increasing amounts (2, 5, 10, 50, 100, 200 or 500 nM) of purified CcaF1. The reactions were run on a 6% native polyacrylamide gel. (B) Predicted structures of the 5′ hairpin in the CcsR1 RNA with and without a mutation of four nucleotides (CCGG→GGCC) in the loop (left side) and gel motility shift experiments with 150 fmol radioactively labelled, mutated CcsR1 RNA incubated with increasing amounts (2, 5, 10, 50, 100, 200 or 500 nM) of purified CcaF1 (right side). The reactions were run on a 6% native polyacrylamide gel.