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. 2021 Mar 12;49(6):3003–3019. doi: 10.1093/nar/gkab146

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — CoIP analysis identifies targets of CcaF1. Analysis of co-immunoprecipitated RNA by RNAseq (Rip-seq) using CcaF1 with 3xFLAG-tag (CcaF1FLAG) or without 3xFLAG-tag (CcaF1, control) in exponential growth phase at 32°C and microaerobic conditions. Read coverage plots from the Integrated Genome Browser display the sequencing reads for selected RNAs. The specific non-coding RNAs and mRNA transcripts of the co-immunoprecipitation were also analyzed by reverse transcription (RT) PCR. The RT-PCR products were separated on a 10% polyacrylamide gel and analyzed by ethidium-bromide staining and are shown below the corresponding read coverage plots.