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. 2021 Feb 28;49(6):3294–3307. doi: 10.1093/nar/gkab110

Figure 4.

Figure 4.

Banf1 interacts with DNA-Dependent Kinase (DNA-PK). (A) DNA-PK was identified as a Banf1 interacting protein via mass spectrometry. A list of significant Banf1-interacting proteins is shown, with a minimum cut-off of 12 peptides. (B) String interactions of significant proteins are shown. (C) Banf1 and DNA-PK are in a complex; Immunoprecipitations from HEK293T cells expressing Flag or Flag-Banf1 at the indicated times post-IR using Flag antibodies. Immunoprecipitates were immunoblotted with the indicated antibodies. (D) Immunoprecipitations from HEK293T cells expressing Flag-Banf1 using DNA-PK antibodies or IgG as a control. Immunoprecipitates were immunoblotted with the indicated antibodies. (E) The histogram represents the analysis of the relative Banf1 binding to DNA-PK at the indicated times post-IR via immunoprecipitation, from (C). (F) Banf1 and DNA-PK directly interact. The recombinant His-Banf1 protein was incubated with purified DNA-PK complex (comprising of DNA-PKcs and Ku70/80) before immunoprecipitation with His beads and immunoblotting with the indicated antibodies. Unless otherwise stated, data shown represent the mean and S.D. of three independent experiments and unpaired t-test was used for statistical analysis: *P < 0.05.