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The Csy:F9 complex binds to DNA non-specifically. (A) The binding of Csy, Csy:F9 and Csy:F1 complexes to dsDNASP and ssDNASP was assessed on EMSA gels. (B) The same experiment shown in (A) was performed with dsDNANS and ssDNANS. (C) Increasing concentrations of Csy or Csy:F9 complex were added to constant concentrations of dsDNASP (left two panels) or dsDNANS (right panel). These experiments were performed using 50 bp DNA fragments 5′-labeled with Cyanine 5 (Cy5).
