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. 2021 Feb 28;49(6):3524–3545. doi: 10.1093/nar/gkab118

Figure 4.

Figure 4.

Alternatively spliced MIG transcripts are not subjected to NMD but bound to polysomes. (A) Agarose gel images showing the nuclear lncRNA Xist as a fractionation control; shown below are alternatively spliced RT-PCR products in the cytoplasmic fraction of the E12 dorsal telencephalon. (B) Schematic of experimental design to isolate RNA bound to polysomes in the U11 cKO Ribotag mouse. (C) Agarose gel images of alternatively spliced RT-PCR products in whole extract (In, input) and bound to polysomes (IP) of E14 control and U11 cKO dorsal telencephalons. (D) Agarose gel images of alternatively spliced RT-PCR products in E12 control and U11 cKO cultured telencephalons with and without NMD inhibitor cycloheximide. The %MSIAS was calculated using ImageJ. (E) Pie chart with the effect of AS on protein domains of affected MIGs. AA = amino acid; CE = cytoplasmic extract; NE = nuclear extract; CHX = cycloheximide. See also Supplementary Figure S7.