Table 1. Macromolecule production information.
| Wild type (WT) | Cysteine to Serine mutant (CS) | |
|---|---|---|
| Souce organism | Prunus persica (L.) Batsch fruit of “Akatsuki” | Prunus persica (L.) Batsch fruit of “Akatsuki” |
| DNA source | cDNA | Gene synthesis and codon optimization |
| Forward primer | ATGAATTCGACTATGGAGGAGGATGGGAAG | AGGGGTATCTCTCGAGAAAAGAGATTACGGTGGTGGT |
| Reverse primer | GCTCTAGAGCGAATTGACCGCCCGAAAATG | AGAAAGCTGGCGGCCGCTTAAAATTGACCACCTGAAA |
| Ligation method | Restriction enzyme and ligation | In-fusion |
| Expression vector | pGAPZαA | pPICZαA |
| Expression host | P. pastoris SMD1168H | P. pastoris KM71H |