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Figure 2. Structural features of β-1,3:1,6-galactan as found in the AGP-subclass. The substitution ratio of β-1,3-galactan backbones by β-1,6-galactan side chains in five vegetables is shown (A). The ratio was calculated based on the proportion of Gal (residue without branching in β-1,3-galactan) and β-1,6-galactooligosaccharides (branching residues in β-1,3-galactan). The distribution of the Gal length of side chains was also calculated (B). The values shown in mol % for Gal, Gal2, Gal3, Gal4, Gal5, and Gal6 of side chains were calculated based on the peak area of ABEE-labelled Gal2, Gal3, Gal4, Gal5, Gal6, and Gal7, respectively. Data are mean values with ±SD (n=3 biological replicates). Representative chromatograms are shown in Supplementary Figure S4.