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PTCR-enabled charge state determination for low-abundance proteins, which cannot be isotopically resolved by high-resolution MS. (a) m/z 3315.9411+ (36.5 kDa), the 5+ charge state of S100-A6 is also detected due to overlap of the (unknown) 11+ precursor with the (S100-A6 dimer) 6+ precursor. (b) m/z 3247.0711+ (35.7 kDa), (c) m/z 3268.259+/11+ (29.4/35.9 kDa) indicated the presence of two overlapping signals within the isolation window. (d) m/z 3632.9710+ (36.3 kDa).
