Skip to main content
. Author manuscript; available in PMC: 2021 Nov 24.
Published in final edited form as: Biochemistry. 2020 Nov 10;59(46):4439–4448. doi: 10.1021/acs.biochem.0c00666

Figure 1.

Figure 1.

NS1 binds to PABP1 as a dimer. (A) Structure of NS1 RBD dimer (blue and yellow) bound to dsRNA (red) (PDB:2ZKO).29 The Arg 35 (magenta) and Arg 46 (green) residues that important for NS1 RBD dimerization are shown. (B) Tricine-PAGE gel of purified WT NS1 RBD and MUT NS1 RBD proteins. (C) Tryptophan polarization of WT NS1-RBD and MUT NS1-RBD. (D) EMSA assay comparing binding of WT NS1 RBD and MUT NS1-RBD to single-stranded RK1 (ssRK1) and double stranded RK1 (dsRK1) RNA. Minus sign indicates no protein was added to the lane. Arrow points to the shifted protein-RNA complex. (E) Anisotropy assay of WT NS1-RBD and MUT NS1-RBD binding to dsRK1 RNA. The final concentration of the RNAs was 10 nM, and the final concentration of NS1 was increased from 0 to 5 μM. The change in anisotropy is shown on the y-axis. The error bars represent the standard deviation from three independent experiments.