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. Author manuscript; available in PMC: 2022 Feb 2.
Published in final edited form as: Anal Chem. 2021 Jan 4;93(4):2694–2705. doi: 10.1021/acs.analchem.0c05126

Figure 1.

Figure 1.

Principle of the glycopeptide signal boosting for secreted glycoprotein analysis. Enriched glycopeptides from secreted glycoproteins in the cell culture media (shown in the deglycosylated form) are labeled with the TMT reagents. When the boosting sample is added, the signal of the deglycosylated peptide in MS1 is increased, thus facilitating the precursor ion selection for MS/MS fragmentation. Higher intensities of the fragments also allow for confident identification of the glycopeptide.