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Optimizing the boosting-to-sample ratio. (A) The number of secreted glycoproteins or deglycosylated peptides from the experiments without the boosting channel, boosted at the 2-to-1 ratio, or boosted at the 10-to-1 ratio. (B and C) Example tandem mass spectra of the deglycosylated peptide KLPPGLLAN#FTLLR from LRG1 from the experiments boosted at the 2-to-1 ratio (B) or boosted at the 10-to-1 ratio (C). The TMT reporter ion region is highlighted and shown in the inset.
