Table 1.
Example Deglycosylated Peptides from Secreted Glycoproteins Quantified from THP-1 Monocytes and Macrophages in Response to LPS or from Hep G2 Cells Treated with TGF-β
| cell | UniProt ID | gene | deglycosylated peptide | site | ModScore | ratio | annotation |
|---|---|---|---|---|---|---|---|
| THP-1 Monocytes + LPS | P98066 | TNFAIP6 | K.N#TSTTSTGNK.N | 258 | 150.77 | 23.10 | tumor necrosis factor-inducible gene 6 protein |
| O15204 | ADAMDEC1 | R.EIKNN#QTEK.H | 61 | 17.01 | 9.58 | ADAM DEC1 | |
| P29460 | IL12B | R.KN#ASISVR.A | 303 | 1000 | 7.51 | interleukin-12 subunit beta | |
| P07996 | THBS1 | K.KVSCPIMPCSN#ATVPD GECCPR.C | 360 | 1000 | 4.11 | thrombospondin-1 | |
| P05362 | ICAM1 | R.LNPTVTYGN#DSFSAK.A | 267 | 127.53 | 2.46 | intercellular adhesion molecule 1 | |
| THP-1 M0 Macrophages + LPS | Q01151 | CD83 | R.N#TTSCNSGTYR.C | 96 | 100.21 | 8.94 | CD83 antigen |
| P98066 | TNFAIP6 | K.N#TSTTSTGNK.N | 258 | 150.77 | 8.34 | tumor necrosis factor-inducible gene 6 protein | |
| P09603 | CSF1 | K.NVFN#ETK.N | 154 | 73.31 | 4.23 | macrophage colony-stimulating factor 1 | |
| P17936 | IGFBP3 | K.VDYESQSTDTQN#FSS ESKR.E | 199 | 1000 | 4.06 | insulin-like growth factor-binding protein 3 | |
| Q9BXX0 | EMILIN2 | K.SLN#DTMHRK.F | 587 | 1000 | 0.62 | EMILIN-2 | |
| Hep G2 + TGF-β | Q14767 | LTBP2 | R.DECWCPAN#STGK.F | 421 | 1000 | 10.60 | latent-transforming growth factor beta-binding protein 2 |
| P19883 | FST | R.CVCAPDCSN#ITWK.G | 124 | 1000 | 3.65 | Follistatin | |
| P15018 | LIF | K.LN#ATADILR.G | 138 | 1000 | 2.12 | leukemia inhibitory factor |
#
refers to the N-glycosylation site.