Skip to main content
. Author manuscript; available in PMC: 2021 Apr 9.
Published in final edited form as: Neuroscience. 2019 May 31;412:60–71. doi: 10.1016/j.neuroscience.2019.05.044

Figure 5: DNMT1 Silencing (siRNA) Effect on Neurosphere Differentiation.

Figure 5:

NPCs from Control (■) and SGA (■) were transfected with non-specific or DNMT1-specific siRNA (20 nM) in differentiating media. (A) Protein expression of Tuj1 and GFAP. (B) Immunostaining of Tuj1 and GFAP. (NS=non-specific siRNA). 5 litters were studied in each group. From each litter, hypothalami from N=4 male pups were pooled representing N=1. F-values: Tuj1=31.36 and GFAP=21.47. * p<0.001 vs non-specific siRNA and c p<0.001 SGA vs Control.