Skip to main content
. Author manuscript; available in PMC: 2021 Apr 9.
Published in final edited form as: Neuroscience. 2019 May 31;412:60–71. doi: 10.1016/j.neuroscience.2019.05.044

Figure 7: Effects of Pharmacologic Inhibitor on NPC Differentiation.

Figure 7:

Control (■) and SGA (■) p1 hypothalamic neurospheres were cultured in differentiating media and treated for 4 days with DNMT1 inhibitor 5-Aza. (A) Protein expression of Tuj1 and GFAP. (B) Immunostaining of Tuj1 and GFAP. (NS=non-specific siRNA). 5 litters were studied in each group. From each litter, hypothalami from N=4 male pups were pooled representing N=1. F-values: Tuj1=52.7 and GFAP=31.62. * p<0.0001 vs non-specific siRNA and c p<0.001 SGA vs Control.