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. 2021 Mar 31;148(7):dev191197. doi: 10.1242/dev.191197

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — SpAxin mRNA is expressed maternally and dynamically throughout embryogenesis. Images show the spatial distribution of Axin mRNA detected by whole-mount RNA in situ hybridization in S. purpuratus eggs and embryos. (A-F) Axin is ubiquitously expressed in the egg (A), and at the 8-cell stage (B), the 16-cell stage (C), 32-cell stage (D), 60-cell stage (E) and 120-cell stage (F). At the 16-cell stage, Axin displays lower expression in the vegetal micromeres (arrow in C). (G) At the hatching blastula stage, Axin mRNA is downregulated in anterior blastomeres. (H,I) By the early gastrula stage, Axin expression is restricted to the vegetal plate (H); by the late gastrula stage, expression is downregulated in the archenteron (I).