Fig. 7.

The GSK-3β-binding domain of Axin is required for rescue of Axin-knockdown embryos. (A,A′) Control embryos. (B,B′) Axin-knockdown embryos. (C,C′) Axin MO and GFP mRNA co-injected embryos. These embryos are posteriorized similar to those injected with Axin MO only (B,B′). (D,D′) Axin MO and Axin mRNA co-injected embryos. These embryos are indistinguishable from control MO-injected embryos (A,A′). (E,E′) Axin MO and Axin ΔDAX mRNA, and (F,F′) Axin MO and Axin Δβcat mRNA co-injected embryos. The Axin-knockdown phenotype is rescued in these embryos. (G,G′) Axin MO and Axin ΔRGS mRNA co-injected embryos. The Axin-knockdown phenotype is rescued in these embryos but when controls are at the pluteus stage, these embryos consistently have defects in the formation of the oral hood. Compare G′ with A′,D′,E′,F′. (H,H′) Axin MO and Axin ΔGID mRNA co-injected embryos. The Axin-knockdown phenotype is not rescued in these embryos. Each experiment was repeated three times. The numbers shown in each panel represent the number of embryos showing the phenotype shown in the panel out of the total number counted in an experiment. Scale bars: 10 µm. Arrowheads indicate the oral hood. Experiments were carried out in L. variegatus.