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. 2021 Mar 10;13(6):8628–8642. doi: 10.18632/aging.202677

Figure 5.

Figure 5

Activation of ATF4 in mice TM induces apoptosis and increase of intraocular pressure. ATF4 adenoviral vectors or GFP adenoviral vectors as control were injected into the anterior chamber of 6~8 weeks old C57BL/6J mice. (A) Visualization of the GFP fluorescence (green) of adenovirus in mice TM after anterior chamber injection for 24 h and increased ATF4 fluorescence (red) could be seen in the ATF4 adenovirus injected eye (Ad-ATF4) compared to the control (Ad-GFP). (B) Intraocular pressure (IOP) of the mice was measured day (left) and night (right) before and after anterior chamber injection. IOP was markedly elevated in the Ad-ATF4 group (n = 8) compared to the control (Ad-GFP) (n = 7) 7 days after the injection, *P<0.05 vs. Ad-GFP. (C) Immunofluorescent staining of the iridocorneal angle showing increased expression of CHOP (red) in the TM of Ad-ATF4 group after anterior chamber injection for 7 days (n = 3). (D) TUNEL staining of the iridocorneal angle 7 days after anterior chamber injection (n = 3). Arrows mark the TM. Blue, nuclear staining with DAPI. BF, bright field. TM, trabecular meshwork. Scale bar, 50 μm.