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. 2021 Mar 6;13(6):9071–9084. doi: 10.18632/aging.202762

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Copyright: © 2021 Kuai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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LncRNA THRIL regulates NF-κB p65 signaling pathway through miR-24-3p, and further regulates OGD/R-induced neuronal apoptosis and inflammatory response. (A) SH-SY5Y cells were transfected and divided into six groups: control, OGD/R, OGD/R+pcDNA3.1-NC, OGD/R+pcDNA3.1-THRIL, OGD/R+NC-mimic, and OGD/R+pcDNA3.1-THRIL+miR-24-3p-mimic groups. (A) Cellular activity was identified by CCK-8. (B) The concentrations of inflammatory factors (IL-6, IL-1β, TNFα) were examined by ELISA. (C) The expression levels of inflammatory factors (IL-6, IL-1β, TNFα) were detected by RT-qPCR. (D) TUNEL was performed to evaluate cell apoptosis. (E) The expression levels of apoptotic proteins were detected by western blot. (F) The p65 protein expression was detected by western blot. Data are shown as mean ± SD for three-independent experiments. **P < 0.01.